亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Differential regulation of the clusterin gene by Ha-ras and c-myc oncogenes and during apoptosis

凝集素 细胞凋亡 生物 程序性细胞死亡 基因表达 细胞生物学 癌基因 信号转导 分子生物学 转染 基因 细胞培养 基因表达调控 癌症研究 细胞周期 遗传学
作者
Gerd Klöck,Stephan Storch,Jens Rickert,Claudia Gutacker,Claudia Koch-Brandt
出处
期刊:Journal of Cellular Physiology [Wiley]
卷期号:177 (4): 593-605 被引量:33
标识
DOI:10.1002/(sici)1097-4652(199812)177:4<593::aid-jcp10>3.0.co;2-f
摘要

Clusterin (ApoJ) is an extracellular glycoprotein expressed during processes of tissue differentiation and regression that involve programmed cell death (apoptosis). Increased clusterin expression has also been found in tumors, however, the mechanism underlying this induction is not known. Apoptotic processes in tumors could be responsible for clusterin gene activation. Alternatively, oncogenic mutations could modulate signal transduction, thereby inducing the gene. We examined the response of the rat clusterin gene to two oncogenes, Ha-ras and c-myc, in transfected Rat1 fibroblasts. While c-myc overexpression did not modify clusterin gene activity, the Ha-ras oncogene produced a seven to tenfold repression of clusterin mRNA; this down-regulation was also observed in the presence of c-myc. Since no induction of the clusterin gene was observed by the two oncogenes, we tested the alternative mechanism involving apoptosis. Growth factor withdrawal induced apoptosis, as shown by DNA degradation and micronuclei formation in the floating cells. Concomittantly we observed a three to tenfold increase in the amount of clusterin mRNA in the adhering cells of Rat1 and the c-myc transformed cell lines, and a weaker induction in the Ha-ras transformed cell line. On the basis of our results, we suggest that clusterin gene induction in the vital cells is produced by signaling molecules that are generated by the apoptotic cells. We conclude that apoptotic processes, not oncogenic mutations, are responsible for increased clusterin expression in tumors.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
Yon完成签到 ,获得积分10
3秒前
8秒前
acc完成签到,获得积分10
9秒前
acc发布了新的文献求助10
13秒前
19秒前
苗条白枫完成签到 ,获得积分10
21秒前
23秒前
学术小白完成签到,获得积分10
24秒前
大橘发布了新的文献求助10
25秒前
学术小白发布了新的文献求助10
27秒前
tkurds完成签到,获得积分10
27秒前
大橘完成签到,获得积分20
30秒前
CATH完成签到 ,获得积分10
30秒前
Nakacoke77完成签到,获得积分10
36秒前
Becky完成签到 ,获得积分10
38秒前
畅快行云完成签到,获得积分20
44秒前
华仔应助jeff采纳,获得10
47秒前
Orange应助eurus采纳,获得10
47秒前
TopBanana完成签到 ,获得积分10
48秒前
锅锅应助学术小白采纳,获得10
53秒前
单薄天宇应助科研通管家采纳,获得10
54秒前
852应助科研通管家采纳,获得10
54秒前
Jasper应助科研通管家采纳,获得10
54秒前
jeff完成签到,获得积分20
57秒前
tkurds发布了新的文献求助10
1分钟前
az完成签到 ,获得积分10
1分钟前
王某人完成签到 ,获得积分10
1分钟前
打打应助默默犀牛采纳,获得30
1分钟前
1分钟前
默默犀牛完成签到,获得积分10
1分钟前
Carrots完成签到 ,获得积分10
1分钟前
1分钟前
rofsc完成签到 ,获得积分10
1分钟前
1分钟前
FashionBoy应助王路飞采纳,获得10
1分钟前
1分钟前
坦率大米发布了新的文献求助10
1分钟前
study关注了科研通微信公众号
1分钟前
eurus发布了新的文献求助10
1分钟前
高分求助中
Les Mantodea de Guyane Insecta, Polyneoptera 2500
Mobilization, center-periphery structures and nation-building 600
Technologies supporting mass customization of apparel: A pilot project 450
China—Art—Modernity: A Critical Introduction to Chinese Visual Expression from the Beginning of the Twentieth Century to the Present Day 430
Tip60 complex regulates eggshell formation and oviposition in the white-backed planthopper, providing effective targets for pest control 400
A Field Guide to the Amphibians and Reptiles of Madagascar - Frank Glaw and Miguel Vences - 3rd Edition 400
China Gadabouts: New Frontiers of Humanitarian Nursing, 1941–51 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3792399
求助须知:如何正确求助?哪些是违规求助? 3336687
关于积分的说明 10281846
捐赠科研通 3053424
什么是DOI,文献DOI怎么找? 1675608
邀请新用户注册赠送积分活动 803581
科研通“疑难数据库(出版商)”最低求助积分说明 761457