ACVR2B型
染色质免疫沉淀
生物
激活素2型受体
分子生物学
转录调控
激活素受体
转录因子
报告基因
基因表达
内分泌学
发起人
基因
转化生长因子β信号通路
转化生长因子
生物化学
作者
Osamu Hashimoto,Kazunari Sekiyama,Tsuyoshi Matsuo,Yoshihisa Hasegawa
出处
期刊:Life Sciences
[Elsevier]
日期:2009-09-01
卷期号:85 (13-14): 534-540
被引量:23
标识
DOI:10.1016/j.lfs.2009.08.007
摘要
Activin E is a newly identified member of the transforming growth factor-β superfamily. To assess the role of activin E in glucose/energy metabolism, we investigated the transcriptional regulation of activin E in the liver. Northern blotting, Western blotting, quantitative real-time polymerase chain reaction (PCR), reporter assays and chromatin immunoprecipitation assays were used in this study. Insulin up-regulated activin E expression at the mRNA and protein level in HepG2 cells. Reporter assays revealed that the putative, functional, promoter sequence of human activin E gene was responsible for the effect of insulin. Mutational analysis of the promoter revealed that CCAAT/enhancer-binding proteins (C/EBPs) play a key role in regulating activin E expression and in the stimulatory effect of insulin on activin E transcription. Chromatin immunoprecipitation assays revealed that the C/EBPs can bind to the activin E promoter in HepG2 cells. The expression of activin E mRNA was up-regulated in the liver of diet-induced obese mice. These observations suggest that activin E plays a pathophysiological role in glucose metabolism.
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