CD3型
流式细胞术
胶原酶
解除
分子生物学
CD11c公司
生物
炎症
消化(炼金术)
化学
抗原
免疫学
酶
生物化学
表型
基因
CD8型
色谱法
作者
John Meenan,D. O. Obradov,Hendrik van Dullemen,G. N. J. Tytgat,S. J. H. Van Deventer
摘要
Mucosal inflammation is associated with altered expression of cell membrane molecules. Disaggregation of tissue for flow cytometry may introduce artefactual changes. In an attempt to prevent the induction of artefacts, cells were fixed prior to isolation. The addition of 0.1% buffered formaldehyde to the collagenase/dispase digestion of mucosal biopsy specimens from patients with inflammatory bowel disease enhances detection of CD3, CD11b, CD16, CD63, and CD14. No significant effect was noted for CD19, CD67 or CD45. The expression of CD3, CD11b and CD45 correlated with the degree of endoscopic inflammation. Dilute buffered formaldehyde may be a useful adjunct to the enzymatic isolation of cells from mucosal specimens, by protecting surface antigens from digestion or alterations in expression.
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