色谱法
化学
质谱法
电喷雾电离
重复性
骨关节炎
脂类学
猎枪
分析化学(期刊)
生物化学
医学
替代医学
病理
基因
作者
Jose Castro‐Perez,Jurre J. Kamphorst,Jeroen DeGroot,Floris P. J. G. Lafeber,Jeff Goshawk,Kate Yu,John P. Shockcor,Rob J. Vreeken,Thomas Hankemeier
摘要
A fast and robust method for lipid profiling utilizing liquid chromatography coupled with mass spectrometry has been demonstrated and validated for the analysis of human plasma. This method allowed quantification and identification of lipids in human plasma using parallel alternating low energy and high energy collision spectral acquisition modes. A total of 284 lipids were identified and quantified (as relative concentrations) in both positive and negative ion electrospray ionization mode. The method was validated with five nonendogenous lipids, and the linearity (r2 better than 0.994) and the intraday and interday repeatability (relative standard deviation, 4−6% and 5−8%, respectively) were satisfactory. The developed lipid profiling method was successfully applied for the analysis of plasma from osteoarthritis (OA) patients. The multivariate statistical analysis by partial least-squares-discrimination analysis suggested an altered lipid metabolism associated with osteoarthritis and the release of arachidonic acid from phospholipids.
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