A Bio‐Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain‐Like Constructs, Using Human Embryonic Stem Cell Derived Neuro‐Progenitors

A bio-acoustic levitational assembly method for engineering of multilayered, 3D brainlike constructs is presented. Acoustic radiation forces are used to levitate neuroprogenitors derived from human embryonic stem cells in 3D multilayered fibrin tissue constructs. The neuro-progenitor cells are subsequently differentiated in neural cells, resulting in a 3D neuronal construct with inter and intralayer neurite elongations.