牛分枝杆菌
生物
蛋白质组
蛋白质亚单位
蛋白质组学
分子生物学
假定蛋白
微生物学
基因
结核分枝杆菌
生物化学
肺结核
医学
病理
作者
Neelja Singhal,Manish Kumar,Divakar Sharma,Deepa Bisht
出处
期刊:Protein and Peptide Letters
[Bentham Science]
日期:2015-11-30
卷期号:23 (1): 51-54
被引量:4
标识
DOI:10.2174/0929866523666151106123121
摘要
BCG, the only available vaccine against tuberculosis affords a variable protection which wanes with time. In this study we have analyzed and compared the proteins which are expressed differentially during broth-culture and intraphagosomal growth of M.bovis BCG. Eight proteins which showed increased expression during the intraphagosomal growth were identified by MALDI-TOF/MS. These were - a precursor of alanine and proline-rich secreted protein apa, isoforms of malate dehydrogenase, large subunit alpha (Alpha-ETF) of electron transfer flavoprotein, immunogenic protein MPB64 precursor, UPF0036 protein, and two proteins with unknown function. Based on these findings we speculate that higher expression of these proteins has a probable role in intracellular survival, adaptation and/or immunoprotective effect of BCG. Further, these proteins might also be used as gene expression markers for endosome trafficking events of BCG. Keywords: Intraphagosomal, MALDI-TOF, M. bovis BCG, proteomics, two-dimensional gel electrophoresis, vaccine.
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