T细胞受体
细胞生物学
化学
生物
免疫学
T细胞
免疫系统
作者
Yu Liu,Wei Wang,Xue-Ru Wu,Xiufang Weng
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 285-293
被引量:4
标识
DOI:10.1007/978-1-0716-0266-9_22
摘要
Mucosal-associated invariant T (MAIT) cells are a novel subset of innate-like T cells that recognize vitamin B metabolites from a range of microbes presented by MHC class I-related molecules (MR1). The term mucosal-associated invariant T cells derives from the fact that MAIT cells are abundant in the liver and mucosal tissues, and human MAIT cells use a semi-invariant TCR Vα7.2 Jα33 paired with Vβ2 or Vβ13. Here, based on the interaction between MAIT cell and its ligand 5-OP-RU/MR1, we describe the protocols for identification, rapid expansion, and isolation of human MAIT cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI