共聚物
马来酸酐
表面改性
材料科学
高分子化学
聚合物
光接枝
烯烃纤维
化学工程
化学
聚合
复合材料
工程类
作者
Yuan Qi,Yindian Wang,Chuxuan Chen,Changwen Zhao,Yuhong Ma,Wantai Yang
出处
期刊:ACS applied bio materials
[American Chemical Society]
日期:2020-04-15
卷期号:3 (5): 3203-3209
被引量:10
标识
DOI:10.1021/acsabm.0c00200
摘要
Immobilization of protein at high efficiency is a challenge for fabricating polymer-based protein chips. Here, a simple but effective approach was developed to fabricate a cyclic olefin copolymer (COC)-based protein microarray with a high immobilization density. In this strategy, poly(maleic anhydride-co-vinyl acetate) (poly(MAH-co-VAc)) brushes were facilely attached on the COC surface via UV-induced graft copolymerization. The introduction of poly(MAH-co-VAc) brushes resulted in an obvious increase in the surface roughness of COC. The functionalized COC showed little reduction in transparency compared with pristine COC, indicating that the photografting treatment did not alter its optical property. The graft density of the anhydride groups on the modified COC could be tuned from 0.46 to 3.2 μmol/cm2. The immobilization efficiency of immunoglobulin G (IgG) on functionalized COC reached 88% due to the high reactivity between anhydride groups and amine groups of IgGs. An immunoassay experiment demonstrated that the microarray showed high sensitivity to the target analyte.
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