3D Immunostaining for CLARITY-processed samples v1

This is a guide for immunostaining CLARITY-processed samples. dx.doi.org/10.17504/protocols.io.8jihuke These steps are meant to be a guide for immunostaining large samples and should be optimized to suit your particular tissues and reagents. Large tissue volumes and dense tissue types will require longer incubation and wash times. The parameters suggested below are for a piece of human thymus tissue approximately 5 mm3 in size. A useful link: http://wiki.claritytechniques.org/index.php/Immunostaining