医学
头孢呋辛
青霉素
血管性水肿
过敏
过敏反应
阿莫西林
免疫球蛋白E
激发试验
抗原
抗生素
药物过敏
免疫学
嗜碱性粒细胞活化
西替利嗪
内科学
皮肤病科
微生物学
病理
抗体
替代医学
嗜碱性粒细胞
生物
作者
García-Avilés C,Miguel A. Sanz,Pedro M. Gamboa,I. Urrutia,I. Antépara,Ignacio Jáuregui,de Weck Al
出处
期刊:PubMed
日期:2005-01-01
卷期号:15 (1): 37-45
被引量:5
摘要
After in vitro allergen-specific stimulation, basophils become activated and release sulfidoleukotrienes LTC4, LTD4 and LTE4. This can be detected by means of the CAST assay. We assessed the positivity criteria and the reliability of antigen-specific sulfidoleukotriene production (CAST) in the in vitro diagnosis of betalactam (BL) allergic patients.We studied a sample of 67 patients (age 48.94 +/- 15.76 years) who had presented with anaphylaxis or urticaria-angioedema within the first 60 minutes after administration of Amoxicillin (54/67), Penicillin G (7/67), Cefuroxime (5/67) or Cefazoline (1/67). All of them had a positive skin test to at least one of the antigenic determinants of Penicillin. As control group 30 adults with negative skin tests who tolerated BL were included. All of them underwent skin tests, oral provocation tests, specific IgE (CAP-FEIA, Pharmacia) and CAST.Positivity criteria were established by means of ROC curves: a sLT release induced by Betalactams of at least 100 pg/ml and greater than or equal to 3 times the basal value. The overall sensitivity of CAST is 47.7% and specificity 83.3%. Sensitivity of specific IgE is 37.8% and specificity 83.3%.We have established validated positivity criteria for the CAST technique in patients allergic to Betalactams. This technique is a useful in vitro diagnostic method in patients with IgE-mediated allergy to Betalactam antibiotics.
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