IN-VITRO CYTOTOXIC ACTIVITY ON ROOTS OF Clerodendrum phlomidis AGAINST NIH 3T3 CELL LINE AND HELA CELL LINE

Summary In this study, cytotoxic activity of Clerodendrum phlomidis crude extracts was investigated. Petroleum ether, chloroform, ethyl acetate and etha nol extracts prepared from roots of Clerodendrum phlomidis were tested for cytotoxic activity on Mouse embryo nic fibroblasts cell line (NIH 3T3) and human cervical cancer cell line (HeLa) using MTT assay. The cells are tested at different concentrations to determine IC 50 (50% growth inhibition) by MTT assay. Chloroform extract shows moderate cytotoxic activit y with an IC 50 value of 148.4 µ g/ml on (NIH 3T3), while Pet ether and ethyl acetate have weak c ytotoxic activity on (NIH 3T3). Ethanol extract has no cytotoxic effect on both NIH 3T3 and HeLa cell line. Ethyl acetate extract has moderate cytotoxic activity with an IC 50 166µg/ml on HeLa cell line, while pet ether and chloroform have weak cytotoxic activity on HeLa cell line.