化学
DNA断裂
细胞凋亡
程序性细胞死亡
流式细胞术
碎片(计算)
免疫印迹
分子生物学
肽
人口
琼脂糖凝胶电泳
细胞周期
亲和层析
细胞
IC50型
DNA
生物化学
体外
生物
酶
人口学
社会学
基因
生态学
作者
Reiko Ikeda,Masaki Kurosawa,Takazumi Okabayashi,Ayako Takei,Masamichi Yoshiwara,Tadashi Kumakura,Norio Sakai,Osamu Funatsu,Akinori Morita,Masahiko Ikekita,Yumi Nakaike,Takeo Konakahara
标识
DOI:10.1016/j.bmcl.2011.06.061
摘要
3-(3-Phenoxybenzyl)amino-β-carboline 2h showed extremely-high activity; the IC50 value was 0.074 μM. To verify 2h-induced cell death types, we observed the chromatin condensation, the DNA fragmentation and activated caspase-3 using Hoechst 33342, agarose electrophoresis and western blot, and suggesting 2h-induced cell death type was apoptosis. Flow cytometry showed that 2h-treated cell was induced SubG1 cell population after G2/M cell cycle arrest. In addition, using affinity chromatography and peptide mass fingerprinting, we found that interacting protein with this compound was α-tubulin protein.
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