化学
尿素
三甲胺
食品科学
组织谷氨酰胺转胺酶
氨
色谱法
生物化学
酶
作者
Sochaya Chanarat,Soottawat Benjakul,Youling L. Xiong
摘要
Abstract Changes in nonprotein nitrogenous compounds and gelling properties of whitecheek shark ( C archarhinus dussumieri ) meat during iced storage for 12 days were monitored. Total volatile base ( TVB ), trimethylamine ( TMA ) and ammonia contents increased, whereas urea content decreased during the storage ( P < 0.05). TVB , TMA , urea and ammonia contents were lower in washed mince, indicating the removal of those compounds by washing. The addition of microbial transglutaminase ( MTG ase, 0.6 unit/g sample) was able to increase breaking force, deformation and water‐holding capacity of both gels from mince and washed mince. Nevertheless, gel strengthening effect of MTG ase was lowered when mince or washed mince were obtained from shark stored for an extended time. Polymerization of myosin heavy chain was enhanced when MTG ase was incorporated but took place at a lower degree for mince and washed mince produced from unfresh shark. Washing process in combination with MTG ase addition could therefore improve gel quality of shark meat. Practical Applications Shark meat is a good source of protein, which could be a suitable source for surimi production. However, the meat is considered to have a low market value because of undesirable odor, which is mainly from the decomposition of urea to ammonia during extended storage. Appropriate washing to remove those compounds, along with the use of microbial transglutaminase ( MTG ase), can be a promising means to improve the textural properties of gels from shark mince. Thus, shark meat can be better exploited, especially for the surimi industry.
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