Apple rootstocks affect functional leaf traits with consequential effects on carbon isotope composition and vegetative vigour

Abstract Composite trees combine optimal traits from both the rootstock and the scion. Dwarfing rootstocks are commonly used to reduce shoot vigour and improve fruit quality and productivity. Although growth habits of different rootstocks have been clearly described, the underlying physiological traits affecting scion vigour are not well understood. Plant water status and stem water potential are strongly influenced by water supply and demand through the soil–plant–atmosphere continuum. In the scion, stomata regulate water loss and are essential to prevent hydraulic failure. Stomatal conductance influences leaf carbon isotope composition. Combined, the effects of reduced stomatal conductance and, consequently, carbon fixation may affect tree growth. These differences could also correspond to differences in scion vigour controlled by rootstock genotype. Here, vegetative growth, gas exchange, stem water potential and leaf δ13C were compared to determine how rootstocks affect scion water relations and whether these differences correspond to shoot vigour. There was a range in vigour among rootstocks by almost 2-fold. Net leaf carbon assimilation rates were lower in rootstocks with lower vigour. Rootstock vigour was closely associated with leaf gas exchange and stem water potential in the scion and was reflected in leaf δ13C signatures. Dwarfing was strongly affected by changes to plant water status induced by rootstock genotype and these changes are distinguishable when measuring leaf and stem δ13C composition. These observations indicate that scion water relations and leaf carbon isotope discrimination were affected by rootstock genotype. These results have implications for better understanding dwarfing mechanisms in apple rootstocks and the relationship with water-use traits.