参考基因
转录组
基因
生物
基因表达
RNA序列
计算生物学
成熟
越桔
遗传学
植物
作者
Feng He,Liangxian Gui,Yan Zhang,Bo Zhu,Xiaoping Zhu,Min Shen,Fengying Wan,Lu Yang,Jiaxin Xiao
出处
期刊:Research Square - Research Square
日期:2022-06-15
标识
DOI:10.21203/rs.3.rs-1759142/v1
摘要
Abstract Background: Vaccinium bracteatum Thunb. (VBT) is widely distributed in the mountainous areas in eastern and southern China. VBT leaves have great medical value and can be used to stain rice to produce “Wumifan”. Its fruits also contain rich nutrients. However, there has been limited attention to exploring the molecular content of VBT. Previously, we performed RNA-seq on three typical VBT fruits that were at various stages of ripening, although a reliable reference gene was lost in validation. Results: In this study, we selected ten candidate reference genes based on previous studies and transcriptomics analyses. Subsequently, these genes were evaluated using a combination of methods, including geNorm, NormFinder, and Bestkeeper, with a comprehensive ranking assessment. As a result, we found that the actin2, NADH, and ADK genes have high reliability for analysing the expression levels of genes involved in fruit development. Furthermore, the transcript levels of 15 DEGs from transcriptomic analysis were assessed using NADH as a reference gene, and RT-qPCR data were highly consistent with the transcriptomic data. Conclusions: These results provide a reliable reference gene for further studying gene expression, which will be beneficial for comprehensively exploring VBT.
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