聚乳酸
羟基烷酸
大肠杆菌
生物化学
代谢工程
生物合成
乳酸
发酵
化学
聚羟基丁酸酯
ATP合酶
溶解
生物
酶
细菌
基因
有机化学
聚合物
遗传学
作者
Mengxun Shi,Mengdi Li,Anran Yang,Miao Xue,Yang Liu,Jagroop Pandhal,Huibin Zou
标识
DOI:10.3389/fbioe.2022.919969
摘要
Polylactic acid (PLA), a homopolymer of lactic acid (LA), is a bio-derived, biocompatible, and biodegradable polyester. The evolved class II PHA synthase (PhaC1 Ps 6-19 ) was commonly utilized in the de novo biosynthesis of PLA from biomass. This study tested alternative class I PHA synthase (PhaC Cs ) from Chromobacterium sp. USM2 in engineered Escherichia coli for the de novo biosynthesis of PLA from glucose. The results indicated that PhaC Cs had better performance in PLA production than that of class II synthase PhaC1 Ps 6-19 . In addition, the sulA gene was engineered in PLA-producing strains for morphological engineering. The morphologically engineered strains present increased PLA production. This study also tested fused propionyl-CoA transferase and lactate dehydrogenase A (fused Pct Cp /LdhA) in engineered E. coli and found that fused Pct Cp /LdhA did not apparently improve the PLA production. After systematic engineering, the highest PLA production was achieved by E. coli MS6 (with PhaC Cs and sulA ), which could produce up to 955.0 mg/L of PLA in fed-batch fermentation with the cell dry weights of 2.23%, and the average molecular weight of produced PLA could reach 21,000 Da.
科研通智能强力驱动
Strongly Powered by AbleSci AI