Biological Control ofEutypa lataon Grapevine by an Antagonistic Strain ofBacillus subtilis

An isolate of Bacillus subtilis from grapevine wood inhibited in vitro growth of Eutypa lata, the causal organism of dieback in grapevines. On potato-dextrose agar, the bacterium caused 91.4% inhibition of mycelial growth of E. lata and 100% inhibition of ascospore germination. Ascospore germination correlated negatively with time of exposure to B. subtilis. Malformation of hyphae of E. lata occured in the presence of B. subtilis. At a concentration of ≥0.8 mg/ml, an antibiotic substance in an ethanol extract from B subtilis totally inhibited germination of ascospores E. lata (...)