体外
化学
水解
细胞培养
核磁共振波谱
生物活性
细胞毒性
细胞毒性T细胞
聚合
衍生工具(金融)
微管
生物化学
立体化学
色谱法
生物
有机化学
细胞生物学
经济
金融经济学
遗传学
聚合物
作者
Israel Ringel,Susan Band Horwitz
出处
期刊:PubMed
日期:1987-08-01
卷期号:242 (2): 692-8
被引量:22
摘要
The hydrolysis products of taxol have been isolated by high-performance liquid chromatography and identified by nuclear magnetic resonance and mass spectroscopy. In contrast to taxol, the major hydrolysis product, baccatin III, has little cytotoxic activity and does not promote in vitro microtubule assembly. In cell culture medium, the concentration of taxol decreases with time and 7-epitaxol, which exhibits properties comparable to those of taxol both on cells and on in vitro microtubuli polymerization, is formed. Baccatin III is found in small quantities in the cell medium, although it is barely detectable within the cells. It is concluded that 7-epitaxol is the major derivative of taxol found in cells and that its presence does not alter, in a major way, the overall biological activity of taxol.
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