Subcellular Localization of PC-1 in LNCaP Prostate Cancer Cells

Objective: To study the subcellular localization of PC-1 in LNCaP prostate cancer cells.Methods: A various of PC-1 segments were amplified and cloned into a eukaryotic expression vector pEGFP-C1,respectively.The sequencing recombinants were transfected into LNCaP cells,observed the distribution of green fluorescence in the cell and validated their expressions by Western blot.Results: The recombinant vectors used for subcellular localization study of PC-1 had been successfully constructed and expressed in LNCaP cell lines.Meanwhile,a sequence that significantly impacts the subcellular localization of PC-1 had been identified.Conclusion: This work provides the basis for further study of subcellular localization and way of functioning of PC-1.