Tissue-specific Expression of the AtJ70 Gene and Subcellular Localization of the AtJ70 Protein in Arabidopsis

With wild type and PAtJ70:GUS transgenic Arabidopsis,tissue-specific expression of Arabidopsis J-domain protein AtJ70 was investigated using GUS staining and real-time PCR.The results show that the AtJ70 gene expresses throughout roots,stems,leaves,flowers and fruits.The expression level of the AtJ70 gene is the highest in flowers and leaves,followed by siliques and roots.The AtJ70 gene has little expression in stems.In addition,with AtJ70-mGFP4 transgenic Arabidopsis,subcellular localization of AtJ70 protein was tested using Confocal.The result shows that AtJ70 mainly targets to the nucleus.