Manufaction of Paraffin Section on Apical Meristem of Soybean

In order to obtain more microstructure information on development of soybean,the methods for apical meristem sections were studied in the paper.Based on the process of fixation,dehydration,transparency,saturated paraffin,embedding,section and dye,straight-sections of the shoot apical meristem of soybean were obtained.The results from this study indicated that the regions splitting actively,such as the top of apical meristem,leaf primordia and flower primordia,were dyed deeply.At the same time,pith meristem zone was dyed lightly.At vegetative growth stage,leaf primordia were found at the basal part of apical meristem,and they became bigger gradually.At reproductive growth stage,the dome-liked apical meristem became longer slightly,then each of floral primordia began to appear in turn.The results could provide the base for further investigation on anatomical structure and developmental biology of shoot apical meristem in soybean.