The Study of Three-dimensional Model of Mouse Blastocysts Implantation

Mouse blastocyst was cultured on human fibrin gel to develop a new three-dimensional embryo invasion model。 Human fibrin gel was found with no influence on the culture of mouse blastocyst . The whole blastocyst retained three-dimensional structure, trophoblast did not flatten but retained a polarized removal and invasion like in vivo . It suggested human fibrin gel could be used as substratum of in vitro three-dimensional model for investigating blastocyst invasive behavior.