Comparative study of two kinds of methods for determination of Polysorbate 80 (excipients) in Huangqi Injection

OBJECTIVE To compare the advantages and disadvantages of the HPLC-ELSD and UV method for determination of Polysorbate 80 in Huangqi Injection.METHODS HPLC-ELSD and UV were used for testing;The HPLC separation was carried out on a molecular gel column,using acetonitrile-water(90:10) as mobile phase.The flow rate was 0.6 mL·min-1,temperature for the drift tube was 110 ℃ and the nitrogen flow rate was 2.3 L·min-1.RESULTS There was a relationship between the results of these two methods,that was to say,the result of UV was about 1.54 times that of HPLC-ELSD.Compared with the result of UV,that of HPLC-ELSD was closer to the theoretical value of Polysorbate 80 In Huangqi Injection.CONCLUSION The HPLC-ELSD was simple,accurate and more conducive to control the content of Polysorbate 80 in Huangqi Injection.