Structure, physicochemical characterization, and antioxidant activity of the highly arabinose-branched exopolysaccharide EPS-M2 from Streptococcus thermophilus CS6

Streptococcus thermophilus CS6 could produce the high exopolysaccharide (EPS) level in optimized skimmed milk medium. However, physicochemical properties and structure of these polymers have not been fully characterized. In this study, two purified fractions (EPS-M1 and EPS-M2) exhibited good rheology, thermostability and antioxidant activity. Further monosaccharide composition, molecular weight and NMR analysis indicated EPS-M2 was composed of galactose, arabinose and glucose (5:2.5:1) with an average molecular weight of 2.22 × 104 Da and its suggested repeating unit was →6)-[α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-α-L-Araf-(1 → 3)]-β-D-Galp-(1 → 6)-[β-D-Galp-(1 → 5)-α-L-Araf-(1 → 5)-{α-L-Araf-(1 → 3)}-α-L-Araf-(1 → 3)]-β-D-Galp-(1→. High EPS production relied on the expression of eps gene cluster and key enzymes of nucleotide sugar metabolism. Overall, EPS-M2 from a potential functional starter S. thermophilus CS6 provided opportunities for natural thickener, stabilizer, and antioxidant agent exploration in the food industry.