p‐Coumaric acid attenuates high‐fat diet‐induced oxidative stress and nephropathy in diabetic rats

Abstract The prevalence of persistent hyperglycaemia during diabetes, impair antioxidant defence system and generate reactive oxygen species, which majorly contribute to its progression and associated complications. Phytochemicals were suggested to scavenge‐free radicals and exert antioxidant effects required to improve insulin sensitivity and reduce the occurrence of diabetes‐associated complications. We hypothesise that a phenolic phytochemical p‐coumaric can reduce diabetes‐induced oxidative stress and improve diabetes‐associated nephropathy in rats. The aim of this study is to analyse the protective effects of p‐coumaric acid against diabetes‐induced oxidative stress and nephropathy in high‐fat diet‐induced diabetic rats. The oral feeding of p‐coumaric acid (20 mg/kg for 12 weeks) was found to significantly decrease the elevated levels of blood glucose in high‐fat diet‐induced type 2 diabetic rats. p‐Coumaric acid treatment also decreases the kidney weight whilst increasing the total body weight of diabetic rats. Furthermore whilst evaluation of the different renal functioning tests, p‐coumaric acid significantly improves histopathological changes and the levels of urea, creatinine and uric acid in serum of diabetic rats, which was otherwise elevated under diabetic conditions. Our results also highlight that p‐coumaric acid is an efficient compound with antioxidant properties and improves the diabetes‐induced change in lipid peroxidation and activities of antioxidant enzymes: catalase, glutathione‐S‐transferase and superoxide dismutase. p‐Coumaric acid thus possesses the potential to prevent diabetic nephropathy by reducing oxidative stress and can thus serve as a potential drug target for pharmaceutical companies.