A Response Rate Matching Dual-Reactable Probe for Fluorescent Recognition of Hydrogen Sulfide

A dual-reactable H 2 S fluorescent probe was designed and synthesized by employing ortho-fluoro-substituted coumarin azide and 7-nitrobenzofurazan-piperazine as the H 2 S reactive groups and the fluorescence quenching groups.The recognition behaviors of the probe to H 2 S were investigated and the results showed that the probe exhibited high selectivity and sensitivity.The fluorescence off-on enhancement was ca.3600-fold, and the detection limit was 4.0×10 -8 mol/L.The results of enzyme activity test indicated that the probe could be used for cystathionine β-synthase (CBS) activity detection and inhibitor screening.Furthermore, the probe was successfully applied for the imaging of H 2 S in living cells.