火球菌属
阿尔戈瑙特
核酸
化学
DNA
劈开
核糖核酸
核苷酸
劈理(地质)
超嗜热菌
分子生物学
生物化学
计算生物学
生物
古细菌
基因
古生物学
断裂(地质)
小干扰RNA
作者
Ruyi He,Longyu Wang,Fei Wang,Wenqiang Li,Yang Liu,Aitao Li,Yuan Wang,Weihua Mao,Chao Zhai
摘要
We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.
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