Humification in extracellular polymeric substances (EPS) dominates methane release and EPS reconstruction during the sludge stabilization of high-solid anaerobic digestion

High-solid anaerobic digestion (HAD) can directly treat dewatered sewage sludge (total solid content ≥15%) with superior volume efficiency. Sludge stabilization during HAD is expected to achieve by throughout organic degradation and conversion towards methane-rich biogas release and humic formation. Sewage sludge is the combination of microbial zoogleas and theirs adsorption of organic and inorganic matter, in which the extracellular polymeric substances (EPS) account 60–80% of total sludge organic matter, inevitably participating most extracellular metabolic pathways. The interactions between EPS transformation and genetically annotated metabolic pathways were found in this research. In brief, noticing the highly cross-linked structures in EPS with major active components of humic substances (HS) and protein (PN), as PN hydrolysis and decomposition in EPS were enhanced in the high-solid anaerobic condition, the exposure of aromatic groups and sites in HS were considerable. HS release was the main factor shifting the electron exchange capacity and activity, which aided in energy metabolism of sludge microorganisms involved in redox reactions, especially the methanogenesis, thus in turn facilitating the PN degradation; Then, the screened humic groups and active protein derives might act as the beneficial precursors to regenerate neo-humic structures, whose significant bridging effect and signal role on stimulating amino acid biosynthesis, member transport and metallic complexation could further contribute to proteolytic condensation and EPS reconstruction. Hence, the in-depth sludge stabilization mechanism during HAD process was established for developing enlightening strategies.