内化
化学
先天免疫系统
纳米载体
单核细胞
纳米材料
免疫系统
THP1细胞系
纳米毒理学
细胞生物学
生物物理学
胶体金
细胞毒性
受体
纳米技术
体外
纳米颗粒
细胞培养
免疫学
毒性
生物化学
药物输送
生物
材料科学
遗传学
有机化学
作者
Sukanya Srijampa,Surachat Buddhisa,Sawinee Ngernpimai,Chanvit Leelayuwat,Siriporn Proungvitaya,Apiwat Chompoosor,Patcharaporn Tippayawat
标识
DOI:10.1021/acs.bioconjchem.9b00847
摘要
The use of gold nanoparticles (AuNP) has been established in nanocarriers, diagnostics, and biosensors. Access to the targeted sites of these nanomaterials could directly involve the first line of defense, the innate immune system. Charges of nanomaterials play a critical role in a number of aspects such as stabilization, cellular uptake, modulation, and function of cells. Interactions and modulations of the charged nanomaterials against the innate immune system may occur even at very low concentration. To understand the effects of charges on monocyte behavior, in this study, the positively and negatively charged AuNP (AuNP+ve and AuNP-ve) of the similar size and shape on cytotoxicity, recognition, cellular behavior, and function were evaluated in vitro using U937 human monocyte cells as an innate immunity model. Both types of AuNP at various concentrations (0–5 nM) exhibited low toxicity. In addition, the cellular internalization of the AuNP+ve and AuNP-ve, as determined by TEM, occurred by different mechanisms, and the internalization had no effect on cellular destruction, as implied by the low levels of %LDH. Interestingly, the AuNP+ve recognition and internalization seemingly entered cells through receptor dependence and strongly affected cellular response to express both pro-inflammatory (IL-1β) and anti-inflammatory (TGF-β) cytokines, while the AuNP-ve stimulated TNF-α expression. Nevertheless, the AuNP-treated cells maintained normal function when exposed to planktonic bacteria. Thus, these results indicated that one part of the immune system interacted with different surface-charged AuNP, suggesting appropiate immunomodulation in biomedicine.
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