生物
穗
突变体
矮化
水稻
油菜素甾醇
甲基磺酸乙酯
侏儒症
叶绿素
拟南芥
遗传学
基因
植物
作者
Yán Li,Renquan Huang,Jiànróng Lǐ,Xiaozhen Huang,Xiaofang Zeng,Degang Zhao
出处
期刊:Phyton-international Journal of Experimental Botany
日期:2021-01-01
卷期号:90 (1): 111-127
被引量:3
标识
DOI:10.32604/phyton.2020.013933
摘要
Plant architecture is a vital agronomic trait to control yield in rice (Oryza sativa L.). A dwarf and small seed 1 (dss1) mutant were obtained from the ethyl methanesulfonate (EMS) mutagenized progeny of a Guizhou glutinous landrace cultivar, Lipingzabianhe. The dss1 mutant displayed phenotypes similar to those of brassinosteroid (BR) deficient mutants, such as dwarfing, dark green and rugose erect leaves, small seeds, and loner neck internode panicles with primary branching. In our previous study, the underlying DSS1 gene was isolated, a novel allele of OsDWARF (OsBR6ox) that encodes a cytochrome P450 protein involved in the BR biosynthetic pathway by MutMap technology. In this work, we confirmed that a Thr335Ile amino acid substitution residing in DSS1/OsDWARF was responsible for the dwarf, panicle architecture, and small seed phenotypes in the dss1 mutants by genetic transformation experiments. The overexpression of OsDWARF in the dss1 mutant background could not only recover dss1 to the normal plant height and panicle architecture but also rescued normal leaf angles, seed size, and leaf color. Thus, the specific mutation in DSS1/OsDWARF influenced plant architecture, seed size, and chlorophyll biosynthesis.
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