人血清白蛋白
圆二色性
化学
聚氯乙烯
焓
荧光
猝灭(荧光)
荧光光谱法
傅里叶变换红外光谱
微塑料
分析化学(期刊)
光谱学
疏水效应
结合常数
核化学
光化学
色谱法
结合位点
立体化学
有机化学
化学工程
生物化学
环境化学
物理
工程类
量子力学
作者
Peng Ju,Yu Zhang,Yifan Zheng,Fenglei Gao,Fenghua Jiang,Jingxi Li,Chengjun Sun
标识
DOI:10.1016/j.scitotenv.2020.139219
摘要
In this study, the interaction of emerging pollutant polyvinyl chloride microplastics (PVC MPs) and human serum albumin (HSA) was investigated by fluorescence spectroscopy, UV-visible (UV-vis) absorption spectroscopy, circular dichroism (CD), and Fourier transform infrared (FT-IR) spectroscopy under simulated physiological conditions. Fluorescence results showed that PVC MPs (about 5000 nm in size) can effectively quench the intrinsic fluorescence of HSA through static quenching owing to the formation of HSA-PVC complex. The binding constants (Ka) between PVC and HSA at different temperatures were calculated as 4.97 × 103 M-1, 3.46 × 103 M-1 and 2.51 × 103 M-1, respectively. The number of binding sites was 1.26. The enthalpy change (ΔH), entropy change (ΔS) and free energy change (ΔG) were calculated to be -59.27 kJ·mol-1, 70.76 J·mol-1 K-1 and - 80.35 kJ·mol-1, respectively, indicating that the interaction of PVC with HSA was mainly driven by electrostatic forces. Moreover, results of UV-vis, FT-IR and CD further demonstrated that the microenvironment and secondary structure of HSA were changed a lot induced by PVC, leading to a decrease in α-helix. This work not only provides an insight into the intermolecular interaction between PVC and HSA, but also elucidates the potential biological toxicity of MPs at a molecular level.
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