电化学发光
乳铁蛋白
化学
紧身衣
信号(编程语言)
衍生工具(金融)
荧光
检出限
光化学
共振(粒子物理)
组合化学
分析化学(期刊)
材料科学
计算机科学
色谱法
光学
物理
生物化学
粒子物理学
经济
程序设计语言
金融经济学
作者
Yao Lü,Kwangjoon Hong,Yang Wang,Yao Zhang,Huan Li,Chusen Huang,Nengqin Jia
标识
DOI:10.1016/j.bios.2020.112664
摘要
A ratiometric electrochemiluminescence resonance energy transfer (ECL-RET) platform depended on novel dye BODIPY derivatives was proposed for rapid detection of lactoferrin. This ECL-RET platform is composed of aptamer decorated BODIPY composites and C60@BSA, in which BODIPY derivative is the ECL probe and can generate significant resonance energy transfer with K2S2O8. BODIPY derivative and K2S2O8 are used as built-in reference signal and calibration signal respectively to eliminate background signal and abnormal change signal by double signal self-calibration process. At the same time, C60, as the accelerator of K2S2O8, can effectively increase the ECL signal and further transfer as much energy as possible to BODIPY derivative. Under optimal conditions, the constructed ECL-RET platform exhibited sensitive detection of lactoferrin in the wide linear range of 10-4- 850 ng/mL with a LOD of 42 fg/mL. Meanwhile, the proposed ECL-RET aptasensor demonstrated superior stability, specificity and reproducibility, displaying favorable application value in practical diagnosis of this method.
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