The expression of thermoTRP channels in the brood patch of jungle fowl (Gallus gallus) during egg incubation

The regulation of egg temperature requires the transfer of heat from the brood patch. Thus, the brood patch needs the presence of thermo receptors as well as an appropriate vasomotor response. During the incubation an exact detection of the egg’s temperature is essential. So, in this study we attempted to detect the presence and regulation of the expressionof thermoTRP channels (thermo Transient Receptor Potential channels) (TRPV1, TRPV3, TRPV4, TRPM8 and TRPA1) during egg incubation. Six incubating Jungle fowl hens, and five non incubating jungle fowl hens and one jungle fowl cock were used as main samples and controls. Total RNA was extracted from liver, kidney, heart, blood, White Blood Cell, Dorsal Root Ganglion and skin. The samples from the skin were taken from the brood patch and inter scapular region. PCR investigation showed that different thermo TRP channels were expressed in different tissues. TRPV1, V3, V4 and M8 mRNA were detected in the skin of brood patch. However, V1 and V3 expression in the brood patch skin did not differ between broody and non broody hens. In conclusion, although considerable morphological changes in the skin of brood patch could be seen, the expression of TRPV1 and V3 channels did not change significantly, but this cannot exclude the alteration in the expression of TRP channels in different stages of broodiness or specific parts of skin like AVAs (Arteriovenous anastomosis) which will be the subject for more studies.