Modifikacija eugenola djelovanjem peroksidaza

The aim of this study was to investigate the effect of peroxidase on eugenol and its modification which results with formation of insoluble compounds that can be easily removed from the clove essential oil. This reaction could be potentially used for the removal of eugenol from various samples. According to the obtained results it can be concluded that peroxidase affects eugenol and causes its modification which has been confirmed using spectrophotometric and chromatographic techniques. The results show that time of reaction between clove essential oil and enzyme is proportional to the degree of eugenol degradation, and that four hour reaction results with almost full disaperance of eugenol from oil sample (degradation of 93%).