干细胞
骨骼肌
细胞生物学
生物
流式细胞术
肌发生
再生(生物学)
心肌细胞
人口
成体干细胞
分离(微生物学)
细胞
分子生物学
细胞分化
解剖
生物化学
生物信息学
医学
基因
环境卫生
作者
Barbara Gayraud-Morel,Francesca Pala,Hiroshi Sakai,Shahragim Tajbakhsh
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 23-39
被引量:18
标识
DOI:10.1007/978-1-4939-6771-1_2
摘要
Isolation of muscle stem cells from skeletal muscle is a critical step for the study of skeletal myogenesis and regeneration. Although stem cell isolation has been performed for decades, the emergence of flow cytometry with defined cell surface markers, or transgenic mouse models, has allowed the efficient isolation of highly enriched stem cell populations. Here, we describe the isolation of mouse muscle stem cells using two different combinations of enzyme treatments allowing the release of mononucleated muscle stem cells from their niche. Mouse muscle stem cells can be further isolated as a highly enriched population by flow cytometry using fluorescent reporters or cell surface markers. We will present advantages and drawbacks of these different approaches.
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