Dose-dependent manner of luteolin in the modulation of spatial memory with respect to the hippocampal level of HSP70 and HSP90 in sleep-deprived rats

Sleep deprivation (SD) induces a variety of deleterious effects on different cognitive functions such as memory. Elevated neuroinflammation, oxidative stress, and apoptosis, and decreased synaptic plasticity and antioxidant capacity are involved in the deleterious effects of SD on memory. On the other hand, luteolin (a flavonoid compound) has antioxidant, neuroprotective, and anti-inflammatory properties. Also, Heat shock protein 70 (HSP70) and Heat shock protein 90 (HSP90) can be involved in modulating memory. In this study, we aimed to assess the effects of SD and luteolin on spatial learning and memory using Morris Water Maze apparatus in rats, with respect to the level of HSP70 and HSP90 in the hippocampus. Luteolin was injected intracerebroventricular (i.c.v.) at the doses of 0.5, 1, and 2 µg/rat. The results showed that SD impaired spatial memory, while luteolin dose-dependently restored SD-induced spatial memory impairment. SD increased the expression level of HSP90 in the hippocampus, whereas luteolin dose-dependently reversed the effect of SD. Furthermore, SD decreased the expression level of HSP70 protein in the hippocampus, while luteolin dose-dependently reversed the effect of SD. In conclusion, HSP70 and HSP90 may be involved in the deleterious effect of SD on memory, and in the improvement effect of luteolin on memory. This is a novel study reporting novel data and we suggest further detailed studies to better understand the interactions between SD, luteolin, and Heat shock proteins.