电化学发光
生物传感器
化学
纳米结构
纳米技术
检出限
DNA
滚动圆复制
组合化学
适体
材料科学
DNA复制
分子生物学
生物化学
色谱法
生物
作者
Linying Yu,Liping Zhu,Peng Yao,Mengting Sheng,Jianshe Huang,Xiurong Yang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-08-04
卷期号:94 (32): 11368-11374
被引量:15
标识
DOI:10.1021/acs.analchem.2c02239
摘要
Achieving rapid and highly sensitive detection of biomarkers is crucial for disease diagnosis and treatment. Here, a highly sensitive and versatile dual-amplification electrochemiluminescence (ECL) biosensing platform was constructed for target detection based on DNA nanostructures and catalyzed hairpin assembly (CHA). Specifically, when the target DNA was present, it would hybridize with the auxiliary strands (D1 and D2) to form an I-shaped nanostructure, which in turn triggered the subsequent catalytic hairpin assembly reaction to generate plenty of double-stranded DNA complexes (H1–H2). The resulting double-stranded complex could be trapped on the electrode surface and adsorbed the ECL signal probe Ru(phen)32+.We found that the I-shaped nanostructure-triggered CHA reaction had higher amplification efficiency compared with traditional CHA amplification. Thus, a sensitive “signal-on” ECL biosensor was constructed for target DNA detection with a detection limit of 1.09 fM. Additionally, by combining the binding properties of C–Ag+–C with an elaborately designed “Ag+-helper” probe, the proposed strategy could be immediately utilized for the highly sensitive and selective detection of silver ions, demonstrating the versatility of the developed biosensing platform. This strategy provided a new approach with potential applications in disease diagnosis and environmental monitoring.
科研通智能强力驱动
Strongly Powered by AbleSci AI