铱
检出限
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
病毒学
逆转录聚合酶链式反应
2019年冠状病毒病(COVID-19)
实时聚合酶链反应
化学
核糖核酸
分子生物学
逆转录酶
荧光
聚合酶链反应
生物
信使核糖核酸
医学
基因
色谱法
生物化学
病理
物理
催化作用
传染病(医学专业)
量子力学
疾病
作者
Ajay Gupta,Tarun Adarsh,Vikas Manchanda,Pijus K. Sasmal,Shalini Gupta
出处
期刊:Dalton Transactions
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:52 (5): 1188-1192
被引量:2
摘要
The highly contagious COVID-19, caused by the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed using reverse transcription polymerase chain reaction (RT-PCR). However, despite being highly sensitive, RT-PCR is also time consuming and quite complex, which limits its use for point-of-care (POC) testing. We have developed a simple single-step fluorescence assay for SARS-CoV-2 RNA detection based on the principle of aggregation-induced emission (AIE) using iridium complexes. Our smartly designed iridium probes fluorescently "turn-on" in the presence of SARS-CoV-2 RNA and give specific results at room temperature within 10 min. The lower limit of detection (LOD) is 1.84 genome copies per reaction, and the sensitivity and specificity of the assay in 20 clinical samples are found to be 90% and 80%, respectively.
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