Intrathecal Delivery of Viruses in a Mouse Model for Treating Central Nervous System Diseases

Source: Li, D., et al. Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice. J. Vis. Exp. (2019)This video demonstrates the procedure for the intrathecal injection of adeno-associated viruses (AAV) into the cerebrospinal fluid (CSF) of an awake mouse, covering syringe preparation, spinal alignment, and precise needle insertion. This method validates targeted gene delivery, supporting its potential for CNS therapies.