Integrative Genomics and Phenotypic Profiling Reveal Lacticplantibacillus plantarum BGI ‐ N6 as a Multifunctional Candidate for Dermatological Probiotic Development

ABSTRACT Probiotics and postbiotics are recognized for their potential to benefit skin health, and complete genome analysis serves as a pivotal tool to accelerate the exploration of probiotic functionalities. To investigate this relationship, this study aimed to elucidate the skin‐promoting potential of Lactiplantibacillus plantarum BGI‐N6 (BGI‐N6) through integrated genomic analysis and phenotypic analysis. Results indicated that the high‐precision complete genome of BGI‐N6 comprises 3 257 641 bases with a 45% GC content and 3030 CDSs, involving multiple genes related to carbon source fermentation, gastrointestinal tolerance, and environmental adaptability. Notably, BGI‐N6 possessed several bacteriostatic, antioxidant, and enzyme activity inhibition‐related genes, which could encode the biosynthetic pathways of bacteriocins, glutathione/thioredoxin antioxidant systems, NADH peroxidase, catalase, and various organic acids. Supporting these genomic insights, in vitro experiments comparative evaluation revealed that BGI‐N6 exhibited superior capabilities relative to Lactiplantibacillus plantarum 299v (299v) in antioxidant activity, inhibition of cutaneous pathogens growth, and suppression of skin‐damaging enzyme (e.g., collagenase and elastase) activity. These effects were correlated with the production of lactic acid, 6‐hydroxyhexanoate, coumarins, and other bioactive compounds by BGI‐N6. The evidence from integrated genomic analysis and in vitro experiments established a critical theoretical foundation for the development of BGI‐N6 as a candidate for probiotic and postbiotic applications targeting skin health.