PI3K/AKT/mTOR通路
医学
肺癌
细胞生长
传统医学
细胞
细胞凋亡
药理学
癌症研究
生物
肿瘤科
生物化学
作者
Felix Y. Feng,P. Hu,Ping Lei,Jun Chen,Xingkui Tao
标识
DOI:10.2174/0113816128296328240329032332
摘要
Background: A classic Chinese medicine decoction, Pinellia ternata (Thunb.) Breit.-Zingiber officinale Roscoe (Ban-Xia and Sheng-Jiang in Chinese) decoction (PZD), has shown significant therapeutic effects on lung cancer. Objective: This study aimed to explore and elucidate the mechanism of action of PZD on lung cancer using network pharmacology methods. Methods: A total of 381 components in PZD were screened, of which 16 were selected as bioactive compounds. The compound-target network consisting of 16 compounds and 79 common core targets was constructed. MTT experiment showed that the PZD extract could inhibit the cell proliferation of NCI-H1299 cells, and the IC50 was calculated as 97.34 ± 6.14 μg/mL. Transwell and wound-healing experiments showed that the PZD could significantly decrease cell migration and invasion at concentrations of 80 and 160 μg/mL, respectively. The in vitro experiments confirmed that PZD had significant therapeutic effects on lung cancer cells, mainly through the PI3K/AKT signaling pathway. Conclusion: PZD could inhibit the cell proliferation, migration, and invasion of NCI-H1299 cells partially through the PI3K/AKT signaling pathway. These findings suggested that PZD might be a potential treatment strategy for lung cancer patients.
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