氧气
氧气增强率
量子产额
生物物理学
化学
氧传感器
活性氧
肿瘤微环境
产量(工程)
极限氧浓度
细胞内
钌
材料科学
纳米技术
生物化学
催化作用
荧光
生物
肿瘤细胞
物理
癌症研究
有机化学
量子力学
冶金
作者
Ashish Kumar,Venkanagouda S. Goudar,Bishal Kumar Nahak,Pei-Hsuan Tsai,Huey-Wen Lin,Fan‐Gang Tseng
出处
期刊:Small
[Wiley]
日期:2023-12-26
标识
DOI:10.1002/smll.202307955
摘要
Unraveling the intricacies between oxygen dynamics and cellular processes in the tumor microenvironment (TME) hinges upon precise monitoring of intracellular and intratumoral oxygen levels, which holds paramount significance. The majority of these reported oxygen nanoprobes suffer compromised lifetime and quantum yield when exposed to the robust ROS activities prevalent in TME, limiting their prolonged in vitro usability. Herein, the ruthenium-embedded oxygen nano polymeric sensor (Ru-ONPS) is proposed for precise oxygen gradient monitoring within the cellular environment and TME. Ru-ONPS (≈64±7 nm) incorporates [Ru(dpp)3 ]Cl2 dye into F-127 and crosslinks it with urea and paraformaldehyde, ensuring a prolonged lifetime (5.4 µs), high quantum yield (66.65 ± 2.43% in N2 and 49.80 ± 3.14% in O2 ), superior photostability (>30 min), and excellent stability in diverse environmental conditions. Based on the Stern-Volmer plot, the Ru-ONPS shows complete linearity for a wide dynamic range (0-23 mg L-1 ), with a detection limit of 10 µg mL-1 . Confocal imaging reveals Ru-ONPS cellular uptake and intratumoral distribution. After 72 h, HCT-8 cells show 5.20±1.03% oxygen levels, while NIH3T3 cells have 7.07±1.90%. Co-culture spheroids display declining oxygen levels of 17.90±0.88%, 10.90±0.88%, and 5.10±1.18%, at 48, 120, and 216 h, respectively. Ru-ONPS advances cellular oxygen measurement and facilitates hypoxia-dependent metastatic research and therapeutic target identification.
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