糖基化
化学
牛血清白蛋白
圆二色性
电场
色谱法
美拉德反应
分析化学(期刊)
生物物理学
生物化学
量子力学
生物
物理
受体
作者
Xindong Xu,Siyao Xiao,Lang‐Hong Wang,Debao Niu,Wenhong Gao,Xin‐An Zeng,Meng Wai Woo,Zhong Han,Ruoyong Wang
标识
DOI:10.1016/j.ijbiomac.2023.128509
摘要
Previous studies demonstrated that the non-thermal effects of pulsed electric fields can promote protein glycation below 40 °C, but it does not always enhance the emulsifying properties of proteins, such as in the bovine serum albumin/glucose model. Therefore, the aim of this study was to investigate the impact of non-thermal effects on the glucose glycation and emulsification properties of bovine serum albumin at 90 °C. The results of circular dichroism, surface hydrophobicity, and molecular dynamics simulations showed that the polarization effect increased the degree of glycation of bovine serum albumin-glucose conjugates from 12.82 % to 21.10 % by unfolding protein molecule, while the emulsifying stability index was increased from 79.17 to 100.73 compared with the control. Furthermore, the results of principal component analysis and Pearson correlation analysis indicated that the ionization effect and the free radicals generated by pulsed electric fields significantly (p < 0.05) inhibited browning and reduced free sulfhydryl content. This study demonstrated that pulsed electric fields combined with heating can prepare glycated proteins with good emulsifying properties in a short period of time and at temperatures lower than conventional heating while reducing energy consumption. This processing strategy has potential applications in improving the emulsifying performance of highly stable proteins.
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