Sustainable transforming toxic sludge into amino acids via bacteria-algae consortium

The utilization of residual sludge by microalgae represents an environmentally sustainable method for resource recovery. In this study, Tetradesmus obliquus was cultured in hydrolysate derived from toxic sludge. Under symbiotic conditions with bacteria, Tetradesmus obliquus demonstrated enhanced toxin degradation capability and biomass accumulation, which exhibited a 1.39-fold increase in algal cell density, a 1.50-fold increase in Rubisco activity, and a total protein content of 341.83 ± 6.99 mg/L on the 30th day of cultivation. Metabolic utilization of substances in the hydrolysate by microalgae led to a toxicity removal rate of up to 60.43% by day 10. Phenylalanine showed the most significant increase among essential amino acids, and transcriptomic profiling identified genes (gene_16399, gene_16602) involved in phenylalanine enrichment. Macrotranscriptomics showed that bacteria upregulated the TCS system and tryptophan metabolism, supplying microalgae with more CO