Quantification of skin penetration of caffeine and propylene glycol applied topically in a mixture by tailored multivariate curve resolution‐alternating least squares of depth‐resolved Raman spectra

The quantitative determination of topically applied substances in the skin is severely limited and represents a challenging task. The porcine skin ex vivo was topically treated with a gel containing caffeine (CF) and propylene glycol (PG), and depth-resolved Raman spectra were recorded with two confocal Raman microscopes. We applied a novel tailored multivariate curve resolution-alternating least squares method to the selected spectral regions (512-604 and 778-1148 cm