适体
纳米孔
乙醇胺
小分子
纳米技术
分子
DNA
化学
材料科学
生物化学
生物
分子生物学
有机化学
作者
Isabel Quint,Jonathan Simantzik,Lars Kaiser,Stefan Laufer,René Csük,David Smith,Matthias Kohl,Hans‐Peter Deigner
标识
DOI:10.1016/j.nano.2023.102724
摘要
In recent decades, nanopores have become a promising diagnostic tool. Protein and solid-state nanopores are increasingly used for both RNA/DNA sequencing and small molecule detection. The latter is of great importance, as their detection is difficult or expensive using available methods such as HPLC or LC-MS. DNA aptamers are an excellent detection element for sensitive and specific detection of small molecules. Herein, a method for quantifying small molecules using a ready-to-use sequencing platform is describe d. Taking ethanolamine as an example, a strand displacement assay is developed in which the target-binding aptamer is displaced from the surface of magnetic particles by ethanolamine. Non-displaced aptamer and thus the ethanolamine concentration are detected by the nanopore system and can be quantified in the micromolar range using our in-house developed analysis software. This method is thus the first to describe a label-free approach for the detection of small molecules in a protein nanopore system.
科研通智能强力驱动
Strongly Powered by AbleSci AI