登革热病毒
表面等离子共振
登革热
背景(考古学)
化学
计算生物学
生物传感器
蛋白质-蛋白质相互作用
小分子
病毒学
纳米技术
生物
生物化学
材料科学
纳米颗粒
古生物学
作者
Yin Hoe Yau,Susana Geifman Shochat
出处
期刊:Methods in molecular biology
日期:2014-01-01
卷期号:: 271-284
被引量:2
标识
DOI:10.1007/978-1-4939-0348-1_17
摘要
Surface plasmon resonance (SPR) biosensors have become the mainstream method for biomolecular interaction analysis. It offers many advantages over conventional methods by its label-free, real-time monitoring, low sample consumption, high throughput, and remarkable sensitivity. We have examined dengue virus protein interactions in the context of antibody affinity measurement, protein–protein interaction, and in the screening of small molecule inhibitors as well as the characterization of the interactions between the small molecule binders and the relevant dengue protein. Here we describe the basic methods involved in performing SPR assays as well as in data processing and evaluation using some examples of dengue proteins.
科研通智能强力驱动
Strongly Powered by AbleSci AI