Effects of x-irradiation on various levels of heterozygosity in maize

Biofilms formed by different bacterial species are likely to play key roles in photocatalytic resistance. This study aims to evaluate the efficacy of a photocatalytic immobilized nanotube system (TiO₂-NT) (IS) and suspended nanoparticles (TiO₂-NP) (SS) against mono- and dual-species biofilms developed by Gram-negative and Gram-positive strains. Two main factors were corroborated to significantly affect the biofilm resistance during photocatalytic inactivation, i.e., the biofilm-growth conditions and biofilm-forming surfaces. Gram-positive bacteria showed great photosensitivity when forming dual-species biofilms in comparison with the Gram-positive bacteria in single communities. When grown onto TiO₂-NT (IS) surfaces for immobilized photocatalytic systems, mono- and dual-species biofilms did not exhibit differences in photocatalytic inactivation according to kinetic constant values (p > 0.05) but led to a reduction of ca. 3-4 log₁₀. However, TiO₂-NT (IS) surfaces did affect biofilm colonization as the growth of mono-species biofilms of Gram-negative and Gram-positive bacteria is significantly (p ≤ 0.05) favored compared to co-culturing; although, the photocatalytic inactivation rate did not show initial bacterial concentration dependence. The biofilm growth surface (which depends on the photocatalytic configuration) also favored resistance of mono-species biofilms of Gram-positive bacteria compared to that of Gram-negative in immobilized photocatalytic systems, but opposite behavior was confirmed with suspended TiO₂ (p ≤ 0.05). Successful efficacy of immobilized TiO₂ for inactivation of mono- and dual-species biofilms was accomplished, making it feasible to transfer this technology into real scenarios in water treatment and food processing.