低温保存
人类受精
体外受精
男科
生物
体外
细胞生物学
胚胎
遗传学
医学
出处
期刊:Methods in molecular biology
日期:2010-10-04
卷期号:: 57-73
被引量:43
标识
DOI:10.1007/978-1-60761-974-1_4
摘要
Cryopreservation of mouse spermatozoa has become the foremost technique for preserving large numbers of different strains of mice with induced mutations. Recently, we have established procedures for cryopreservation of mouse spermatozoa and in vitro fertilization using cryopreserved spermatozoa to obtain a relatively high fertilization rate. This chapter attempts to show these procedures in simple terms.
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