Studies On Immobilized Enzymes For Biosensor Applications

In the context of the usage of immobilized enzymes for sugar biosensor applications, the present investigations attempt to a better understanding of the various aspects of the enzyme applications. The work has involved the construction of an immobilized enzyme based biosensor for glucose and sucrose in food and fermentation analysis, study of the effect of various parameters on the performance of the biosensor and also basic biochemical aspects of the immobilized single and multienzyme systems with respect to the effect of temperature, pH, denaturants as well as operational stability. Aspects considered include: Construction and optimization of biosensor, Estimation of sucrose in the presence of glucose, Stability of single and multienzyme systems, Thermal stability studies of immobilized enzymes using biosensor, Mechanism of thermal inactivation of GOD, Reversible denaturation of the immobilized single and multienzyme systems,